Fig 2. S. senegalensis sperm viability after cryopreservation.

A) Three different internal cryoprotectants (CPAs) were tested: dimethyl sulfoxide (DMSO), dimethyl formamide (DMF) and methanol (MetOH), at 10% concentration, supplemented with two different external CPAs: 1% bovine albumin serum (BSA) and 10% egg yolk (EY). The percentage of viable cells was expressed as a mean of percentages ± SE of 8–10 pools of 10 individual males for each treatment. One-way ANOVA (p<0.05) was performed and no significant (p< 0.05) differences were found among the cryopreservation media. B) Flow cytometry histograms obtained after propidium iodide (PI) staining.