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. 2017 Oct 20;12(10):e0186542. doi: 10.1371/journal.pone.0186542

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© 2017 Riesco et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Three different internal cryoprotectants (CPAs) were tested: dimethyl sulfoxide (DMSO), dimethyl formamide (DMF) and methanol (MetOH), at 10% concentration, supplemented with two different external CPAs: 1% bovine albumin serum (BSA) and 10% egg yolk (EY). The percentage of fragmented DNA (DNAt) was expressed as a mean of percentages ± SE of 6–10 pools of 10 individual males for each treatment. One-way ANOVA (p<0.05) was performed and no significant (p< 0.05) differences were found among the cryopreservation media. A total of 100 cells were analyzed in the two slides performed by treatment.