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. 2017 Oct 20;12(10):e0186542. doi: 10.1371/journal.pone.0186542

Table 1. List of primers for S. senegalenesis transcript abundance assays.

Gene name, abbreviation, sequences start from 5´ to 3´, reference, annealing temperature and amplicon size are specified for each pair of primers.

GENE REFERENCE ABBREVIATION OLIGO SEQUENCE 5´-3 TM (°C) AMPLICOM LENGHT (BP)
Heat shock protein 70 kDa protein [30] hsp70 F-GCTATACCAGGGAGGGATGGAAGGAGGG 60 119
R-CGACCTCCTCAATATTTGGGCCAGCA
Heat shock protein 90 kDa protein cDNA variant AA [31] hsp90AA F-GACCAAGCCTATCTGGACCCGCAAC 60 105
R-TTGACAGCCAGGTGGTCCTCCCAGT
Heat shock protein 90 kDa protein cDNA variant BB [31] hsp90BB F-TCAGTTTGGTGTGGGTTTCTACTCGGCTTA 60 148
R-GCCAAGGGGCTCACCTGTGTCG
Glutathione peroxidase [30] gpx F-GATTCGTTCCAAACTTCCTGCTA 63 212
R-GCTCCCAGAACAGCCTGTTG
β-actin [33] actb2 F-AATCGTGACCTCTGCTTCCCCCTGT 60 114
R-TCTGGCACCCCATGTTACCCCATC
Translation initiation factor eIF-α subunit [33] ef1α F-GATTGACCGTCGTTCTGGCAAGAAGC 60 142
R-GGCAAAGCGACCAAGGGGAGCAT
Glyceraldehyde-3-phosphate dehydrogenase [33] gapdh2 F-AGCCACCGTGTCGCCGACCT 60 107
R-AAAAGAGGAGATGGTGGGGGGTGGT
18s RNA [33] 18s F-GAATTGACGGAAGGGCACCACCAG 60 148
R-ACTAAGAACGGCCATGCACCACCAC
Ubiquitin [33] ubq F-AGCTGGCCCAGAAATATAACTGCGACA 60 93
R-ACTTCTTCTTGCGGCAGTTGACAGCAC