Table 1. Prediction of vulnerability of T164I variation by computational programs and calculation of total structural deviation.
| Prediction programs for disease related mutations | Score | Prediction | |
|---|---|---|---|
| SIFT | 1 | tolerated | |
| Polyphen2 | 0.008 | benign | |
| PANTHER | 0.114 | neutral | |
| PhD-SNP | 0.188 | neutral | |
| SNAP | 0.245 | neutral | |
| Meta-SNP | 0.326 | neutral | |
| MAPP | 85% | neutral | |
| Predict SNP | 74% | neutral | |
| SDM | SASA prediction Wild = 58.3% T164I variant = 41.9% |
Pseudo ΔΔG = 2.79 Kcal/mol | Prediction: destabilizing and cause protein malfunction |
| STRUM | ΔΔG = 1.47 Kcal/mol | ||
| iMUTATNT3.0 Prediction Effect: Decrease in Stability, ΔΔG = 1.23 Kcal/mol | |||
| Total Structural RMSD (Å) = 5.63 | |||