Fig 6. Various substrates rely differently on Herp and Ubc6e for degradation.

(A–D) RD cells stably expressing SHH-FLAG (A), NHK-FLAG (B), TTR D18G-FLAG (C), and NS1κLC-FLAG (D) were transfected with control siRNA and siRNA targeting Ubc6e or Herp. At 36 h post-transfection, cells were mock infected (−) or infected (+) with EV71 (MOI = 10) for 12 h and then treated with (+) or without (−) CHX for an additional 4 h. The cells were then harvested and cell lysates were analyzed by western blotting with antibodies against FLAG, Herp, Ubc6e, 2C, and actin. (E) RD cells were treated as described in (A–D) and western blotting was performed using antibodies against CD147, Herp, Ubc6e, 2C, and actin. The graphs show the quantification of the relative substrate (lower panel). The data are presented as means ± SD of two independent experiments.