The fold increase of mRNA expression in DRGs of SCI mice compared to normal spinal intact mice. P2X2, P2X3, TRPA1, and TRPV1 were overexpressed in SCI mice compared to spinal intact rats (control). Compared to group A, P2X3 and TRPA1 in groups B and C were decreased, and P2X2 was decreased in groups B with statistical significances. The ratio of each marker to GAPDH mRNA was used for statistical analyses. The fold increase of each marker expression was calculated using the average values of each group. *P < 0.05 versus Group A (once daily bladder squeeze) using one-way ANOVA with Dunnett's post hoc test. †P < 0.05 versus normal spinal intact mice using Student t-test