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. 2017 Aug 27;8(43):73407–73418. doi: 10.18632/oncotarget.20562

Figure 2. ∆Np63α drives the ECS cell phenotype.

Figure 2

A. B. C. SCC-13 cells were double electroporated with control- or ∆Np63α-siRNA and seeded for spheroid formation, invasion and migration assay. The values are mean ± SEM and the asterisks indicate a significant reduction (n = 3, p < 0.01). D. E. ECS cells were electroporated with empty vector (EV) or ∆Np63α expression vector, and seeded for spheroid formation and invasion assays in the presence of 0 or 20 μM SFN. The single asterisk indicates a significant reduction in SFN treated as compared to untreated control cultures. The double asterisks indicate a significant increase as compared to the SFN treated group (n = 3, p < 0.01). F. Cells, treated as indicated, were grown in non-attached conditions for 5 days and lysates were prepared for immunoblot.