Table 1. Primers used for RT-PCR amplification.
Gene | Primers | Length (bp) | Access No. |
---|---|---|---|
IFITM3 | F: 5’- TGGTGGGAGACATCATTGGG-3’ R: 5’- GAAAATTACCAGGGAGCCAGTG-3’ | 134 | NM_001201382.1 |
IFNα | F: 5’- TGGTGCATGAGATGCTCCA-3’ R: 5’- GCCGAGCCCTCTGTGCT-3’ | 55 | XM_021062981.1 |
IFNβ | F: 5’- TCGCTCTCCTGATGTGTTTC-3’ R: 5’- TTCTGACATGCCAAATTGCT-3’ | 94 | NM_001003923.1 |
p38 | F: 5’- CTTACGGATGACCACGTTCAGT-3’ R: 5’- GCTCACAGTCTTCATTCACAGC-3’ | 127 | XM_005318767.3 |
TNFα | F: 5’- AACCCTCTGGCCCAAGGA-3’ R: 5’- GGCGACGGGCTTATCTGA-3’ | 57 | NM_214022.1 |
TBK1 | F: 5’- ACAGATTTTGGTGCAGCCAG-3’ R: 5’- CCTTATTCCTACGTGGCCCT-3’ | 229 | XM_021090854.1 |
NFκB | F: 5’- CCCATGTAGACAGCACCACCTATGAT-3’ R: 5’- ACAGAGGCTCAAAGTTCTCCACCA-3’ | 132 | NM_001048232.1 |
TLR4 | F: 5’- TCAGTTCTCACCTTCCTCCTG-3’ R: 5’- GTTCATTCCTCACCCAGTCTTC-3’ | 166 | NM_001293316.1 |
GAPDH | F: 5’- TGGTGAAGGTCGGAGTGAAC-3’ R: 5’- GGAAGATGGTGATGGCCTTTC-3’ | 369 | XM_021091114.1 |