Skip to main content
. 2017 May 15;8(43):73757–73765. doi: 10.18632/oncotarget.17890

Figure 2. Reintroduction of human PKCβ in cultured cells restores RB (Ser780) phosphorylation with no significant effect on its abundance.

Figure 2

PKCβ-/- hepatocytes were isolated from twelve-week-old C57BL/6J male mice as described previously (15). The recombinant adenovirus expressing green fluorescent protein GFP and GFP-PKCβ were used to transiently overexpress PKCβ as described previously (15, 29). After 36 h infection with 1 to 100 multiplicity of infection (MOI), cells were harvested with ice-cold PBS. Immunoblotting were used to compare phospho- and total RB levels. Quantitation of bands is shown in the right panel. The results shown are representative of at least three separate experiments. The detection of a weak band in the first two lanes possibly reflects slight cross-reactivity of the PKCβ antibody with other endogenous PKC isoforms, particularly PKCδ.