Table 1. Th-related gene expression in mouse colonic mucosa detected by real-time PCR.
| Gene | Control | NCTC11639 | TNBS | TNBS+NCTC11639 |
|---|---|---|---|---|
| IL-12p35 | 1 ± 0.06 | 0.90 ± 0.06 | 1.35 ± 0.06 | 0.63 ± 0.08* |
| TNF-α | 1 ± 0.06 | 0.93 ± 0.14 | 2.76 ± 0.44 | 1.23 ± 0.10* |
| IFN-γ | 1 ± 0.08 | 0.91 ± 0.08 | 1.59 ± 0.01 | 0.32 ± 0.01** |
| STAT-1 | 1 ± 0.01 | 0.97 ± 0.01 | 2.26 ± 0.04 | 1.44 ± 0.02** |
| IL-23p19 | 1 ± 0.34 | 1.10 ± 0.26 | 2.16 ± 0.01 | 1.81 ± 0.01** |
| IL-17A | 1 ± 0.15 | 1.22 ± 0.24 | 5.15 ± 0.22 | 1.11 ± 0.03* |
| IL-6 | 1 ± 0.25 | 0.89 ± 0.35 | 5.79 ± 0.54 | 0.61 ± 0.02* |
| IL-1β | 1 ± 0.52 | 0.85 ± 0.14 | 30.27 ± 10.66 | 0.86 ± 0.19* |
| TGF-β | 1 ± 0.06 | 1.21 ± 0.09 | 1.82 ± 0.15 | 1.21 ± 0.15* |
| STAT-3 | 1 ± 0.03 | 0.93 ± 0.05 | 3.47 ± 0.12 | 0.67 ± 0.08** |
| IL-4 | 1 ± 0.02 | 1.96 ± 0.03 | 0.25 ± 0.03 | 4.71 ± 0.20* |
| IL-5 | 1 ± 0.03 | 1.57 ± 0.04 | 0.27 ± 0.04 | 0.85 ± 0.01* |
| IL-10 | 1 ± 0.20 | 1.17 ± 0.20 | 0.32 ± 0.03 | 0.82 ± 0.01** |
| STAT-6 | 1 ± 0.08 | 1.21 ± 0.08 | 0.18 ± 0.01 | 0.26 ± 0.02* |
Relative mRNA levels are normalized to β-actin and expressed as a fold relative to the Control groups. Data are shown as the mean ± SD from 10 mice per group. *P < 0.05,**P < 0.01 vs TNBS groups.