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. 2017 May 19;8(43):73892–73904. doi: 10.18632/oncotarget.18029

Figure 4. Metformin inhibited angiogenesis in peri-necrotic region by impeding HIF-1α-induced expressions of pro-angiogenic factors.

Figure 4

4T1 tumors extracted from untreated or metformin-treated mice were embedded in paraffin and cut into 4–5 μm for IHC staining. (A) IHC staining for CD31, HIF-1α, Ang-1, Ang-2, PDGF-B, PlGF, VEGF and FGF-2 in untreated or metformin-treated BALB/c mice. Scale bar: 100 μm. Necrotic region is indicated by “NR” and its boundary is surrounded by red dotted line. (B) Quantification of IHC scores (addition of intensity score and positive signal area) of angiogenesis-associated factors, including HIF-1α, Ang-1, Ang-2, PDGF-B, PlGF, VEGF and FGF-2 (n = 10). Quantitative data are indicated as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns indicates no significant (P > 0.05).