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. 2017 May 16;8(43):73905–73924. doi: 10.18632/oncotarget.17879

Figure 6. The BCL-2 family proteins are crucial for the DHS-induced apoptosis in IMR32 cells.

Figure 6

(A) Silencing the BCL-2 family proteins. (B) and (C) Effect of BCL-2 family proteins on the DHS-induced apoptosis. apoptosis. The SCR and KD cells were generated by siRNA/shRNA techniques and the levels of the target proteins assessed by immunoblotting the respective whole cell extracts, using suitable antibodies against the above proteins. The protein bands were detected using a Kodak Gel-doc software and the intensity ratios of the individual bands to that of respective vehicle control, taken as 1 (arbitrary unit) were quantified after normalizing with respective loading controls. The respective SCR and KD cells were incubated with different concentrations (0-40 μM)of DHS for 48 h, and the sub-G1 populations analyzed by flow cytometry. The protein bands were detected using a Kodak Gel-doc software and the intensity ratios of the individual bands to that of respective vehicle control, taken as 1 (arbitrary unit) were quantified after normalizing with respective loading controls. All determinations were made in duplicates for immunoblots and five replicates for flow cytometry analyses in 3-4 different experiments. The values are mean ± S. E. M. *p<0.05 compared to vehicle control; $p<0.05, #p<0.01 compared to respective SCR cells. Representative images and histograms are shown.