Skip to main content
. 2017 May 22;8(43):73947–73963. doi: 10.18632/oncotarget.18166

Figure 1. HSF1 and AKT are co-activated in multiple breast cancer subtypes.

Figure 1

(A) Lysates from the indicated cell lines were subjected to immunoblotting with the indicated antibodies. (B) p-HSF1 and p-AKT bands from (A) were quantified by ImageJ and correlated using Pearson correlation. (C) A cohort of 50 breast tumors was subjected to immunohistochemistry for the indicated antibodies. H-scores were calculated as described in the Materials and Methods and the scores for p-HSF1 (S326) and p-AKT (S473) were analyzed with Pearson correlation. (D) Table below is count of tumors within the cohort (n = 50) that were positive for p-AKT (S473) and p-HSF1 (S326) across breast cancer subtypes. (E) Gene set enrichment analysis (GSEA) was performed as described in Materials and Methods using a gene signature for AKT activity. The expression database contained 664 breast cancer patients publicly available from GEO. (F) Using the same database from (D), AKT activity score was calculated using the same signature from (E) and was correlated with Hsp70, Hsp90, and Slug.