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. 2017 May 22;8(43):73947–73963. doi: 10.18632/oncotarget.18166

Figure 3. Loss of HSF1 activity reduces growth of metastatic breast cancer cells.

Figure 3

(A) Lysates from MDA-MB-231 parental and metastatic cell lines were subjected to immunoblotting with indicated antibodies. (B) Cells were transfected with control or HSF1 siRNA followed by immunoblotting. (C) MCF7 cells were transfected with empty vector, HSF1, or HSF1 + HSF1-S326A followed by luciferase assay using a Slug promoter reporter. (D–E) Cells with and without HSF1 knockdown from (B) were subjected to anchorage-dependent (D) or anchorage-independent (E) colony assays. (F–G) Cells were transfected with empty vector or HSF1-S326A followed by anchorage-dependent (F) or anchorage-independent (G) colony assays. *Indicates significant difference (p < 0.05).