Figure 2. Treatment of lung cancer cells with demethylating agent, 5-Aza-dC, results in the demethylation of the NY-ESO-1 promoter and consequential mRNA and protein re-expression.

NY-ESO-1 hypermethylated lung cancer cell lines A549 and NCI-H460 were treated with or without 5-Aza-dC for 72 hr at the indicated doses. Results obtained from (A) qMS-PCR (N = 3, mean with SD), (B) qRT-PCR (N = 2, mean with SD, perform in triplicates) or (C) IHC analysis (representative image) were shown.