Skip to main content
. 2017 May 23;8(43):74058–74067. doi: 10.18632/oncotarget.18202

Figure 2. Changes in CD70 protein- and mRNA expression levels in response to CDDP-therapy.

Figure 2

(A) A549 cells were treated with vehicle or CDDP (3.5 µM, 7 µM or 13 µM) for 24 h. CD70 expression levels were determined 1 h, 6 h, 24 h and 48 h after treatment. Graph represents ∆ mean fluorescence intensity (∆MFI) of three independent experiments; (B) NCI-H1975, A549 and NCI-H1650 cells were treated with vehicle or CDDP (7 µM) for 24 h. Graph represents ∆MFI of three independent experiments. In addition, representative histogram plots of vehicle- and CDDP-treated cells are displayed; (C) CD70 expression was measured by immunofluorescence (red) after treatment with vehicle or CDDP (7 µM) for 24 h in the A549 cells. Nuclei were stained by DAPI (blue); (D) CD70 mRNA levels were determined 24 h after treatment with vehicle or CDDP (7 µM). Graph represents relative mRNA levels in comparison to vehicle-treated cells. *P < 0.05, significant increase compared to vehicle.