Figure 2. Functional analysis of SNPs and candidate genes associated with prostate cancer progression.
(A) Expression quantitative trait locus associations of rs9393682 and rs1378033 with nearby genes (HIST1H1C and HFE for rs9393682; SGCZ and TUSC3 for rs1378033) expression in prostate tissues (GTEx dataset). Numbers in parentheses indicate the number of cases. (B) Effect of 1,25-VD on candidate genes expression. PC-3 cells were treated with vehicle or 1,25-VD for indicated time points. Total mRNA was prepared and the corresponding gene expression was determined by qRT-PCR. Values represent the fold change in gene expression relative to vehicle-treated control. Asterisk indicates significant difference (P < 0.05). (C) Correlation of candidate genes expression with prostate cancer aggressiveness. Numbers in parentheses indicate the number of cases. (D) Kaplan-Meier analysis of progression-free survival based on candidate genes expression using an independent dataset from Sboner et al. Numbers in parentheses indicate the number of cases.