Skip to main content
. 2017 Jun 9;8(43):74188–74208. doi: 10.18632/oncotarget.18431

Figure 6. Depleted expression of TFF3 decreased 3D Matrigel growth of trastuzumab resistant BT474 cells, without re-sensitization to trastuzumab.

Figure 6

(A) Control and trastuzumab resistant BT474 cells were transiently transfected with the indicated concentrations of scrambled or TFF3 siRNA. TFF3 protein levels were measured with β-ACTIN as input control. (B) Control and trastuzumab resistant BT474 cells transfected with either scrambled or TFF3 siRNA were cultured in 5% FBS media containing 4% matrigel, and treated with 10 µg/ml trastuzumab over a period of 8 to 10 days. Cell viability in 3D Matrigel was measured by AlamarBlue assay. Ctrl: control cells; Tras Res: trastuzumab resistant cells. Scale bar, 200 µm. *p < 0.05; NS, no significance.