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The mRNA levels of fibrosis markers (A) TGFβ1 and (B) fibronectin in kidney tissues were measured using real-time PCR. Paraffin-embedded kidney sections were stained with (C and D) picrosirius red stain, (E and F) anti-αSMA (1:200), and (G and H) anti-collagen IV (1:200) antibodies. Original magnification: 200×; scale bar: 100 μm. Data are presented as means ± SE of 5 mice/group; *p < 0.05 vs. control, †p < 0.05 vs. DM.
