Figure 1. Inhibition of cyclooxygenase 2 (COX-2) expression by auranofin in PC-3 cells.
(A-B) Cells were treated with the indicated concentration of auranofin for 24 h. (A) Expression of COX-2 mRNA was determined using qPCR. (B) COX-2 proteins were measured using western blot analysis. Quantification of western blots (n = 3). (C-D) Cells were treated with 1 μM auranofin for 12, 24, or 48 h. (C) Expression of COX-2 mRNA was determined using real-time qPCR. (D) COX-2 proteins were measured using western blot analysis. Quantification of western blots (n = 3). *, #P < 0.05.