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(A-D) PC-3 cells were transfected with scrambled or annexin A5 siRNA for 48 h. (A) Expression of COX-2 mRNA was determined using qPCR. (B) COX-2 proteins were measured using western blot analysis. Quantification of western blots (n = 3). (C) PGE2concentration in cell supernatants was measured by ELISA. (D) COX-2 promoter activity was determined using dual luciferase assay. *, #P < 0.05.
