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. 2017 Aug 10;8(43):74406–74422. doi: 10.18632/oncotarget.20160

Figure 3. JNK signalling mediates TNC regulation of cell migration and invasion.

Figure 3

(A) A western blot analysis was performed to test the phosphorylated and total levels of JNK and c-Jun in the Capan-2, AsPC-1 and PANC-1 cells transfected with siTNC or siControl. (B) The total and phosphorylated protein levels of JNK and c-Jun were analysed by western blot in Capan-2, AsPC-1 and PANC-1 cells after treatment with TNC plasmid or control vector. (C) The expression levels of p-JNK and p-c-Jun were normalized to the total JNK and c-Jun levels. (D) PANC-1 cells were preincubated with SP600125 or DMSO for 1 h before exogenous TNC stimulation. Cells were harvested at the indicated time, and the levels of total and phosphorylated JNK and c-Jun were analysed by western blot. (E) PANC-1 cells were preincubated with SP600125 for 1 h before transfection with TNC plasmid or control vector. Then, the cell migration and invasion capacities were assessed using Transwell assays. Data represent the mean ± SD. (n = 3, *P < 0.05).