Skip to main content
. 2017 Aug 12;8(43):74582–74594. doi: 10.18632/oncotarget.20216

Figure 1. Effects of BPIS on LPS induced inflammation.

Figure 1

(A) Cells were incubated with or without BPIS (0.6 mg/mL) in the presence of LPS (0.01 μg/ml) for 24 h. The levels of secreted TNF-α, IL-1β, IL-6, IL-8 and IL-10 at 24 h in the cell culture medium were analyzed by ELISA. Data were presented as mean ± SEM (n=4, **p<0.01 vs. control, # p<0.05 vs. LPS). (B) Total RNAs were prepared from HT-29 cells. Cells were treated with 0.01 μg/ml LPS for 24 h, and then stimulated with or without 0.6 mg/ml BPIS for 24 h. The mRNA expression levels of IL-1β, IL-6, IL-10 and TNF-α were carried out by RT–PCR and expression levels were normalised to GAPDH. Data were presented as mean ± SEM (n=3,* p<0.05 vs. control, **p<0.01 vs.control, # p<0.05 vs. LPS, ## p<0.01 vs. LPS). (C) Cells were cultured with different concentrations of BPIS (0, 0.4 mg/ml and 0.6 mg/ml) in the absence or presence of 0.01 μg/ml LPS for 24 h. The protein expression levels of IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by western blot. Data were presented as mean ± SEM (n=3, *p<0.05 vs. control, # p<0.05 vs. LPS, ## p<0.01 vs. LPS)