Figure 1. IGFBP-2 increases in response to different glucose levels.

MCF-7 cells were treated with 5, 9, and 25mM glucose for 48h and the abundance of secreted (A) and endogenous (B) IGFBP-2 was examined by Western immunoblotting. The optical densitometry measurements of IGFBP-2 secreted (C) and in lysates (D) in MCF-7 cells. T47D cells were treated with 5, 9, and 25mM glucose for 48h and the abundance of secreted (E) and in lysates (F) IGFBP-2 was examined by Western immunoblotting. The optical densitometry measurements of IGFBP-2 secreted (G) and in lysates (H) in T47D cells. (I) MCF-7 cells were seeded at 0.4x10⁶ cells in 5mM glucose growth medium for 24h prior to switching to SFM of different glucose concentrations 5, 9, and 25mM glucose for 24h. The cells were lysed with Trizol and the level of IGFBP-2 mRNA expression was determined by real-time PCR. (J) T47D cells were treated the same way as MCF-7 cells in 5, 9, and 25mM glucose for 24h, and the level of IGFBP-2 expression was determined by real-time PCR. 18S was used a internal housekeeping gene. The graphs represent the mean±SEM of three independent repeats each conducted in triplicate.