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. 2017 Aug 16;8(43):74635–74648. doi: 10.18632/oncotarget.20287

Figure 5. Nuclear ERs increase IGFBP-2 levels that is protective against chemotherapy.

Figure 5

(A) MCF-7 cells were seeded at 0.1x106 cells in 5mM growth medium then were serum starved for 24h in 5mM and 25mM glucose SFM. The cells were treated with 17β-estradiol for 48h and doxorubicin was introduced in the last 24h of incubation. Trypan blue exclusion assay was used to determine the level of cell death. (B) Western immunoblotting showing changes in IGFBP-2 and FASN protein abundance in 5mM and 25mM glucose in the presence and absence of 17β-estradiol. Optical densitometry measurements of IGFBP-2 (C) and FASN (D) in response to 17β-estradiol in 5mM and 25mM glucose. The graphs represent the mean±SEM of three independent experiments each performed in triplicate.