Figure 7. The involvement of the FASN/ERα/Akt pathway in hypoxia regulation of IGFBP-2.

(A) MCF-7 cells were seeded at 0.1x10⁶ cells in 5mM growth medium. The cells were then treated in 5mM and 25mM glucose and were exposed to hypoxia. Changes in the abundance of HIF-Iα, FASN, ERα, Akt and p-Akt were analysed using Western immunoblotting. Densitometry measurements of changes in the abundance of FASN (B) ER (C) p-Akt/Akt (D) under normal and hypoxic conditions in 5mM and 25mM glucose. The graphs represent the mean±SEM of three independent repeats each conducted in triplicate.