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. 2017 Aug 16;8(43):74649–74660. doi: 10.18632/oncotarget.20301

Figure 3. Fasting induces cancer cell autophagy, downregulates CD73 expression of cancer cells and suppresses adenosine generation in vitro.

Figure 3

(A) The mRNA expression of Atg5 was examined by qPCR in normal or fasting medium for 24 h or 48 h. **P<0.01. (B) The expression of Atg5 was tested by Western blotting. (C) The expression of LC3I/II was tested by Western blotting. (D) The mRNA expression of CD73 was tested by qPCR. *P<0.05. (E) The expression of CD73 was determined by Western blotting. (F) The levels of adenosine were tested by ELISA. *P<0.05, **P<0.01.