Skip to main content
. 2017 Aug 24;8(43):75038–75049. doi: 10.18632/oncotarget.20500

Figure 1. H3R agonist immethridine could alleviate the severity of EAE.

Figure 1

(A) EAE mice were treated with immethridine (15 mg/kg) every other day via i.p. from day 8 post-immunization. Control groups were given PBS injection. Data are mean±SD (n=6). **p<0.01 versus vehicle. (B) H&E staining and (C) LFB staining of paraffin sections of spinal cords. After the mice (n=6) were sacrificed on day 21, the spinal cord samples were isolated from vehicle and immethridine–treated EAE mice and fixed in 4% (w/v) paraformaldehyde for 48h, then the paraffin-embedded sections were stained with hematoxylin-eosin or luxol fast blue. Scale bar, 100 μm. (D) RT-PCR analysis of the pro-inflammatory cytokines of splenocytes. The splenocytes from vehicle and immethridine–treated EAE mice were collected after the mice were sacrificed on day 21 and the total RNAs were extracted. RT-PCR was performed with the specific primers of cytokines and the PCR products were analyzed on a 1% agarose gel stained with ethidium bromide. (E) Q-PCR analysis of the pro-inflammatory cytokines of splenocytes. **p<0.01, ***p<0.001 versus vehicle. Data are representative of three independent experiments.