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. 2017 Aug 24;8(43):75114–75126. doi: 10.18632/oncotarget.20540

Figure 2. TRPM8 is functionally expressed in mouse differentiated white adipocytes.

Figure 2

(A) RT-PCR analysis of the expression of Trpm8 and β-actin using mouse differentiated white adipocytes with (RT (+)) and without (RT (-)) reverse transcription (RT). Control (Ct.) lanes indicate the results with each plasmid DNA as a template. (B) Results of real-time RT-PCR analysis of Trpm8 expression using mouse pre-adipocytes, 4-day-differentiated adipocytes and 8-day-differentiated adipocytes. mRNA expression levels were normalized to that of the ribosomal protein gene (36B4), a housekeeping gene un-affected by adipogenesis. Data are presented as mean ± SEM, n = 6. (C) Western blot result of TRPM8 protein in mouse differentiated white adipocytes. (D) Changes in intracellular Ca2+ concentration ([Ca2+]i) in mouse white adipocytes response to a TRPM8 agonist, 500 μM menthol. Five μM ionomycin was used to confirm cell viability.