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. 2017 Sep 8;8(43):75284–75297. doi: 10.18632/oncotarget.20777

Figure 5. The inhibitory role of HSP90AA1-IT1 in the biological functions of miR-885-5p in the glioma cells.

Figure 5

(A) The U87MG and U251 glioma cells were transfected with miR-885-5p mimic alone or together with a lentiviral vector expressing HSP90AA1-IT1. The growth curves of the transfected cells were shown using CCK8 assay. Data were presented as mean ± s.d. from three independent experiments. The U87MG glioma cells were transfected with miR-885-5p mimic alone or together with a lentiviral vector expressing HSP90AA1-IT1. Cell proliferation (B) and invasion (D) were determined by EDU staining assay and transwell assay, respectively. The results represent mean ± s.d. from three independent experiments. **P < 0.01 vs. NC. (C) Both cell lines were transfected with miR-885-5p mimics. Apoptosis were tested by duel-staining with Annexin V and PI followed by flow cytometry assay. Data were presented as mean ± s.d. from three independent experiments. *P < 0.05 vs. NC, ***P < 0.001 vs. NC. (E) The U87MG glioma cells were transfected with miR-885-5p mimic. The mRNA levels of the representative EMT markers including E-cadherin, N-cadherin and Vimentin were measured by qRT-PCR. Each bar represents mean ± s.d. from three independent experiments. *P < 0.05 vs. NC. Scale: 20μm.