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. 2017 Sep 8;8(43):75284–75297. doi: 10.18632/oncotarget.20777

Figure 6.

Figure 6

(A) The potential binding sequences of CDK2 and miR-885-5p as well as the mutated sequences were shown. (B) Relative luciferase activities were determined by dual-luciferase reporter assay. Data were presented as mean ± s.d. from three independent experiments. ***P < 0.001 vs. NC. (C) The U87MG cells were transfected with the lentiviral vectors expressing sh-HSP90AA1-IT1 or wild-type HSP90AA1-IT1. The protein levels of CDK2 were measured by Western Blotting analyses. GAPDH was used as an internal control. (D) The U87MG glioma cells were transfected with miR-885-5p mimic alone or together with a lentiviral vector expressing HSP90AA1-IT1. CDK2 expressions were determined by Western Blotting analyses. GAPDH was used as an internal control. (E) The U87MG cells were transfected with a lentiviral vector expressing sh-HSP90AA1-IT1 alone or together with miR-885-5p inhibitor. HSP90AA1-IT1-NC and miR-885-5p-NC are their respective controls. CDK2 expressions were determined by Western Blotting analyses. GAPDH was used as an internal control. Data are presented as the mean ± s.d. from three independent experiments.