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. Author manuscript; available in PMC: 2018 Oct 19.
Published in final edited form as: Cell. 2017 Sep 21;171(3):615–627.e16. doi: 10.1016/j.cell.2017.08.048

Fig. 1. Fibril formation by the low-complexity domain of FUS.

Fig. 1

(A) Human FUS-LC sequence. Experiments were performed on samples with an additional N-terminal His tag, bearing the sequence MSYYHHHHHHDYDIPTTENLYFQGAMDP. (B) TEM image of negatively-stained FUS-LC fibrils. (C) AFM image of FUS-LC fibrils adsorbed to mica, with fibril heights indicating diameters of 5.5 ± 0.7 nm. Inset shows the height profile along the dotted red line. (D) Dark-field TEM image of unstained FUS-LC fibrils. Tobacco mosaic virus (TMV) particles are included as mass-per-length (MPL) standards for image intensity calibration. (E) MPL histogram obtained from multiple dark-field images. Red line is a Gaussian fit, centered at 50 kDa/nm, with 11 kDa/nm full-width-at-half-maximum. Vertical dashed line indicates the MPL value expected for a single cross-β structural unit with 0.48 nm intermolecular spacing. See also Figs. S1 and S7.