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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Hepatology. 2017 Sep 29;66(5):1616–1630. doi: 10.1002/hep.29309

Figure 4. Bmp inhibition after hepatocyte ablation increases BEC number in regenerating livers via proliferation.

Figure 4

(A) Experimental scheme illustrating the stages of Mtz and DMH1 treatment. Quantification of the total numbers of BECs in regenerating livers treated with DMH1 for three different time-windows. (B) Confocal projection images showing Tp1:H2B-mCherry and Tp1:mAGFP-gmnn expression in regenerating livers. Arrows point to mCherry/mAGFP-gmnn double-positive cells. Quantification of the percentage of mAGFP-gmnn+ cells among H2B-mCherry+ BECs. (C) Confocal projection images showing the expression of Alcam, Tp1:H2B-mCherry, and fabp10a:CFP-NTR in regenerating livers at R30h. Quantification of the total numbers of BECs (H2B-mCherry+/Alcam+) per liver. (D) Confocal projection images showing EdU labeling and Alcam and Tp1:H2B-mCherry expression in regenerating livers. Arrows point to EdU+ BECs. Quantification of the percentage of EdU+ cells among BECs. Scale bars: 20 μm; error bars: ±SEM.