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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Apr 20;141(1):257–268.e6. doi: 10.1016/j.jaci.2017.03.032

Figure 1.

Figure 1

Effect of Dexamethasone (Dex) on IL5+ ILC2s. PBMCs from an allergic asthmatic patient were cultured with Aspergillus, IL2/IL33 or IL2/IL7/IL33 for 5 days with and without Dex. A: PBMCs were stained with labeled antibodies against the lineage markers, CRTH2, IL7Rα, and their isotype antibody controls (shown at the top). Live and lineage-negative cells were gated for CRTH2+ and IL7Rα+ cells. Dual CRTH2+IL7Rα+ cells were then analyzed for IL5 expression. B: Expression of IL5 in lin-CRTH2+IL7Rα+ cells in cultures with Aspergillus, IL2/IL33 or IL2/IL7/IL33 in presence or absence of Dex. C: Effect of Dex on BAL lin-CRTH2+IL7Rα+IL5+ cells. BAL cells from an allergic asthmatic patient was cultured with and without Dex for 5 days and then analyzed for live CD45+lin-CRTH2+IL7Rα+IL5+ cells. D & E: Cumulative data on the effect of Dex on blood (D) and BAL (E) lin-CRTH2+IL7Rα+IL5+ cells 14 allergic asthmatic patients. V: vehicle; Dex: Dexamethasone