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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Apr 20;141(1):257–268.e6. doi: 10.1016/j.jaci.2017.03.032

Figure 4.

Figure 4

A–D: Effect of Dex on CRTH2 and IL7Rα expression on ILC2s. PBMCs were cultured with vehicle or Dex for 5 days and then stained for flow cytometry. Live lin- cells were gated for CRTH2 and IL7Rα. A representative flow cytogram is shown in A. Cumulative data from 14 asthmatic patients for the frequency of CRTH2+ and IL7Rα+ cells are shown in B & C, respectively. D shows the mean fluorescence intensity of IL7Rα+ expression from the same study subjects. E: Comparison of the frequency of IL7Rα+ ILCs between blood and BAL. PBMCs and BAL cells from asthmatic patients were studied ex vivo for IL7Rα on gated live lin-CRTH2+ cells. Each symbol represents a donor (n=24). F: Effect of Dex on TSLP signaling. PBMCs from allergic asthmatic patients were cultured with vehicle (V) or Dex for 3 days and stimulated IL2/TSLP for increasing period of time. The frequency of pSTAT5+ cells on gated live lin-CRTH2+ cell population was measured. P*<0.05, N=4, paired t test).