Table 1.

Multiplex PCR-CGE techniques for detection of GMO.

Multiplexing	Techniques	Target genes	Reference
4-plex	Taxon specific	LEC, Zein	Burrell et al., 2011
	Element specific	p35S, tNOS
5-plex	Taxon specific	ADH	Holck and Pedersen, 2011
	Event specific	GA21, MON810, NK603, Bt11	Nadal et al., 2009
6-plex	Taxon specific	acp1	Guo et al., 2011
	Event specific	Bollgard, Bollgard II, RR, 3006-210-23, 281-24-231
9-plex	Element specific	bar, chy, pAct, CP4-EPSPS, Cry1Ab	Guo et al., 2011
	Event specific	GT73, OXY235
9-plex	Taxon specific	HMG	Heide et al., 2008b
	Event specific	T25, GA21, TC1507, MON863, MON810, NK603, Bt176, Bt11

Zein, Zein gene obtained from corn; LEC, soybean lectin gene; p35S, 35S cauliflower mosaic virus promoter; tNOS, nopaline synthase gene terminator; ADH, alcohol dehydrogenase I gene obtained from corn; Chy, chymopapain gene of papaya; acp1, acyl carrier protein 1 gene of cotton; bar, phosphinothricin-N-acetyltransferases gene of Streptomyces hygroscopicus; HMG, major high-mobility group protein gene of corn; pAct, rice actin gene promoter section; CP4-EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase gene of Agrobacterium tumefaciens strain; Cry, gene containing Bacillus thuringiensis δ-endotoxin.