Table 5.
Representative examples indicating NGS strategies targeting transgenic event.
| NGS approaches | NGS platforms | Target genes | Target sizes | Reference |
|---|---|---|---|---|
| Targeted sequencing | 454 systems (Roche | Bt11 | 324 bp | Song et al., 2014 |
| Applied Science) | CP4-EPSPS | 498 bp | ||
| LEC | 118 bp | Song et al., 2014 | ||
| p35S | 195 bp | Song et al., 2014 | ||
| PacBio RS (Pacific | vip3Aa2 from | 150 bp to | Song et al., 2014 | |
| Biosciences) | MIR162 | 2 Kbp | Liang et al., 2014 | |
| Whole genome Sequencing | LLRICE62 rice | 385 Mbp | Wahler et al., 2013 | |
| Bt rice | 385 Mbp | Willems et al., 2016 | ||
| HiSeq (Illumina) | TT51-1 rice | 385 Mbp | Yang et al., 2013 | |
| MON87704 soybean | 1115 Mbp | Yang et al., 2013 | ||
| MON17903 soybean | 1115 Mbp | Kovalic et al., 2012 |
CP4-EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain; LEC, soybean lectin gene; p35S, 35S cauliflower promoter; VIP3A, vegetative insecticidal protein 3A.