FIGURE 2.

Transcriptomic analysis of genes involved in cellular development and whose expression differs between ΔmtrA and wild-type M145. (A) chp and rdl genes (involved in formation of the hydrophobic sheath of the aerial mycelium); (B) ram genes (involved in production of SapB); (C) developmental regulatory genes of the bld and whi families. Total RNA was extracted from mycelia of M145 and ΔmtrA grown for 72 h at 30°C on YBP solid agar, cDNA libraries were prepared, and transcript levels were determined by RNA-Seq. The value for a specific gene was calculated as the log₂ ratio of the expression level in RPKM (reads per kilobase transcriptome per million mapped reads) of the gene in ΔmtrA relative to that in M145, with red indicating an increase in transcript abundance in ΔmtrA and blue indicating a decrease.