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. 2017 Oct 16;8:2013. doi: 10.3389/fmicb.2017.02013

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Copyright © 2017 Zhang, Wu, Zhu, Liu, Wang, Cao, Chen, Tao and Pang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The developmentally regulated expression of chaplin (A) and rodlin (B) genes in S. coelicolor M145 is blocked in ΔmtrA. Expression of these morphogen genes was determined by real-time PCR analysis. M145 and ΔmtrA were grown on YBP solid medium, and RNA samples were isolated at the indicated times. Expression of hrdB, encoding the major sigma factor in M145, was used as an internal control. For each gene, the expression level in M145 at 36 h was arbitrarily set to one. The y-axis shows the fold change in expression levels in M145 (gray bars) and ΔmtrA (light bars) over the levels in M145 at 36 h. Results are the means (± SD) of triplet experiments.