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. 2017 Oct 16;8:2018. doi: 10.3389/fmicb.2017.02018

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Copyright © 2017 Águila-Arcos, Álvarez-Rodríguez, Garaiyurrebaso, Garbisu, Grohmann and Alkorta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of plasmids from 25 staphylococcal clinical isolates by agarose gel electrophoresis after digestion with nuclease S1. One microgram of plasmid DNA from each isolate was digested with 30 U of nuclease S1 at 37°C for 45 min. After digestion, the plasmids were analyzed by 1% (w/v) agarose gel electrophoresis in 1 × TAE buffer. Lanes 1–25: digested plasmid DNA from each strain (lane numbers correspond to the number of the isolate). Lanes M: DNA molecular weight marker 1 kb Plus DNA Ladder. Bands corresponding to plasmids are indicated with arrows.