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Detection of traK gene in 25 clinical isolates by PCR (A) and Southern blotting (B). Amplicons of traK gene (573 bp) were visualized on 1% (w/v) agarose gels. Lanes 1–25: clinical isolates. Lanes +: positive control. Lanes −: negative control. Lanes M1: DNA molecular weight marker 1 kb Plus DNA Ladder. Lanes M2: DNA molecular weight marker VI DIG-labeled.
