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. 2017 Oct 16;8:2018. doi: 10.3389/fmicb.2017.02018

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Copyright © 2017 Águila-Arcos, Álvarez-Rodríguez, Garaiyurrebaso, Garbisu, Grohmann and Alkorta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of pre_pT181 gene in 25 clinical isolates by PCR (A) and Southern blotting (B). Amplicons of pre_pT181 gene (397 bp) were visualized on 1% (w/v) agarose gels. Lanes 1–25: clinical isolates. Lanes +: positive control. Lanes −: negative control. Lanes M1: DNA molecular weight marker 1 kb Plus DNA Ladder. Lanes M2: DNA molecular weight marker VI DIG-labeled.