Table 5.
Isolate | Antibiotic resistance phenotypea | Antibiotic resistance genotypeb | Transfer genes | Plasmidsc | Biofilm-forming capacity 24 hd | |
---|---|---|---|---|---|---|
<20 kb | >20 kb | |||||
1 | ERY | ermB, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traE, traG, traK, traL, traM* | 1 | 0 | 0 |
2 | GEN | ermB, ermC, ermG, tetK, aac6-aph2a | prepSK41, prepT181, traG, traL | 0 | 2 | 1 |
3 | ERY | ermC, tetK, aac6-aph2a | prepSK41, prepT181, traL* | 0 | 0 | 1 |
4 | ERY | ermB*, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traL | 1 | 0 | 0 |
5 | – | ermB, ermC, tetK*, aac6-aph2a* | prepSK41, prepT181, traK, traL | 0 | 2 | 3 |
6 | ERY, GEN | ermB, ermC, tetK, aac6-aph2a | prepSK41, prepT181, traG, traK, traL | 1 | 1 | 2 |
7 | ERY, GEN | ermB, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traE, traK, traL | 0 | 3 | 2 |
8 | GEN | ermC, tetK*, aac6-aph2a | prepSK41, nespSK41, prepT181, traG, traK | 0 | 2 | 3 |
9 | ERY, TET | ermC, tetK* | prepSK41*, prepT181, traL* | 4 | 3 | 2 |
10 | ERY | ermB, ermC, tetK, aac6-aph2a, vanB | prepSK41, prepT181, traG, traL | 0 | 1 | 2 |
11 | – | ermB, ermC, tetK | prepSK41, nespSK41, prepT181, traE, traG, traL, traM | 0 | 3 | 1 |
12 | ERY, GEN, TET | ermC, tetK, aac6-aph2a | prepSK41*, prepT181, traG*, traL | 2 | 3 | 3 |
13 | GEN | ermB, ermC, tetK, aac6-aph2a | prepSK41, prepT181, traE*, traG*, traK, traL*, traM* | 1 | 2 | 2 |
14 | - | ermC, tetK*, aac6-aph2a | prepT181, traE, traG*, traK, traL | 0 | 2 | 0 |
15 | ERY | ermB, ermC, ermG*, tetK, aac6-aph2a | prepSK41, prepT181, traL* | 1 | 1 | 1 |
16 | ERY | ermB, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traE*, traG*, traK, traL | 1 | 0 | 0 |
17 | ERY | ermB, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traE, traG, traK, traL, traM | 0 | 1 | 2 |
18 | – | ermB*, ermC, tetK, aac6-aph2a | prepSK41, nespSK41, prepT181, traE, traG, traK, traL | 0 | 1 | 0 |
19 | – | ermC, tetK, aac6-aph2a | prepSK41, prepT181, traK*, traM | 0 | 2 | 2 |
20 | ERY, GEN | ermB*, ermC, tetK, aac6-aph2a | prepSK41, prepT181, traE*, traG*, traK*, traL, traM | 1 | 1 | 2 |
21 | ERY, GEN | ermB*, ermC, tetK | prepSK41, prepT181, traE, traG, traK, traL, traM | 1 | 1 | 2 |
22 | – | ermB, ermC, tetK, aac6-aph2a | prepSK41, prepT181, traE*, traG*, traL, traM | 1 | 2 | 3 |
23 | ERY, GEN | ermC, aac6-aph2a | prepSK41, prepT181, traE*, traG, traL | 0 | 2 | 2 |
24 | GEN | ermB*, ermC, aac6-aph2a | prepSK41, prepT181, | 0 | 3 | 2 |
25 | ERY, GEN | ermB, ermC, tetK, tetM, aac6-aph2a | prepSK41, prepT181, traG*, traK*, traL, traM | 0 | 1 | 3 |
GEN, gentamicin; ERY, erythromycin; TET, tetracycline.
aac6-aph2a, gentamicin; ermB/ermC/ermG, erythromycin; tetK/tetM, tetracycline; vanB, vancomycin resistance genes.
Numbers indicate the number of plasmid bands observed in the 1% agarose gel or in the PFGE.
0, no biofilm-forming capacity; 1, weak biofilm-forming capacity; 2, strong biofilm-forming capacity, 3, very strong biofilm-forming capacity.
Weak signal intensity in the Southern blot.