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Regulation of T6SS1 genes by OpaR. The qRT-PCR (A), primer extension (B), LacZ fusion (C), and EMSA (D) were done as Figure 2. (E) DNase footprinting assay. Labeled coding or non-coding DNA probes were incubated with increasing amounts of purified His-OpaR, and subjected to DNase I footprinting assay. The footprint regions were indicated with vertical bars.
