Table 1.
Oligonucleotide primers used in this study.
| Target | Primers (forward/reverse, 5′–3′) | Reference |
|---|---|---|
| Construction of mutants | ||
| aphA | GTGACTGCAGCGCAGCAAATAACCAGAC/CCAATCACTTCAAGTTCTGTTGTCTTCAATCCAAATGGTC | Sun et al., 2012 |
| GACCATTTGGATTGAAGACAACAGAACTTGAAGTGATTGG/GTGAGCATGCGTTTTCGTGACCGCTGTG | ||
| GTGACTGCAGCGCAGCAAATAACCAGAC/GTGAGCATGCGTTTTCGTGACCGCTGTG | ||
| opaR | GTGACTGCAGACTGCCTTGGTAACGCTCTG/GTTCGTGTTCAAATCTGAGCTATCCATTTTCCTTGCCATTTG | Zhang et al., 2012 |
| CAAATGGCAAGGAAAATGGATAGCTCAGATTTGAACACGAAC/GTGAGCATGCATGGGCTGCATCAGGTCG | ||
| GTGACTGCAGACTGCCTTGGTAACGCTCTG/GTGAGCATGCATGGGCTGCATCAGGTCG | ||
| toxR | GTGACTGCAGAAACGCAATTTGTCTGATG/ATCTTCATGCTGGCCTCCTTTAGTTCTTCTTAGATGGATGATG | This study |
| CATCATCCATCTAAGAAGAACTAAAGGAGGCCAGCATGAAGAT/GTGAGCATGCAATTCGGCGGCTTTGTTC | ||
| GTGACTGCAGAAACGCAATTTGTCTGATG/GTGAGCATGCAATTCGGCGGCTTTGTTC | ||
| Construction of complemented mutants | ||
| aphA | GATTCTAGAAGGAGGAATTCACCATGTCATTACCACACGTAATC/GACAAGCTTTTAACCAATCACTTCAAGTTC | Sun et al., 2012 |
| opaR | GATTCTAGAAGGAGGAATTCACCATGGACTCAATTGCAAAGAG/GACAAGCTTTTAGTGTTCGCGATTGTAG | Zhang et al., 2012 |
| toxR | GATTCTAGAAGGAGGAATTCACCATGACTAACATCGGCACCAA/GACAAGCTTTTATTTGCAGATGTCTGTTGG | This study |
| Protein expression | ||
| aphA | AGCGGGATCCATGTCATTACCACACGTAATC/AGCGAAGCTTTTAACCAATCACTTCAAGTTC | Sun et al., 2012 |
| opaR | AGCGGGATCCATGGACTCAATTGCAAAGAG/AGCGAAGCTTTTAGTGTTCGCGATTGTAG | Zhang et al., 2012 |
| toxR | AGCGGGATCCATGACTAACATCGGCACCAA/AGCGAAGCTTTTAAGGATTCACAGCAGAAG | This study |
| qRT-PCR | ||
| VP1388 | CGTCCTTACACCTGATGAG/TGTCGAATAGCCGTTAG | This study |
| hcp1 | GGTCAACCTACTGGTCAACG/TAGTGCTCTTGCTTGCCTTG | This study |
| VP1400 | GTATTAGACACGTTGCCATC/ATCTGCTTGCCTCATTCG | This study |
| VP1409 | TTCTGTGCTCGACTTGTG/TTCAGTGTACTCAACCATCC | This study |
| Primer extension | ||
| VP1388 | / GATAGCTCGTTGGAGGAAAG | This study |
| hcp1 | / GAGTTTCACCGTTGATAGAC | This study |
| VP1400 | / ACACTTTGCACTCTTTTATGG | This study |
| VP1409 | / TTAGACAATAAAAAGCCGA | This study |
| LacZ fusion | ||
| VP1388 | AAAGTCGACCAATGGTGAATATGCCGTG/AAGGTACCGATAGCTCGTTGGAGGAAAG | This study |
| hcp1 | GCGCGTCGACGCTATCGGGTGTAGACGCTG/GCGCGAATTCGAGTTTCACCGTTGATAGAC | This study |
| VP1400 | GAGGTCGACTTTTGCCGAAGAAATAC/TATAGAATTCGCCAATCTTTGCTCGTTCAG | This study |
| VP1409 | ATATGTCGACAACACATGGCATAAATGAGTCC/CCCGAATTCTCTTCTTGTGAAGTCGCTGAA | This study |
| EMSA | ||
| VP1388 | CAATGGTGAATATGCCGTG/GATAGCTCGTTGGAGGAAAG | This study |
| hcp1 | GCTATCGGGTGTAGACGCTG/GAGTTTCACCGTTGATAGAC | This study |
| VP1400 | TTTTGCCGAAGAAATAC/GCCAATCTTTGCTCGTTCAG | This study |
| VP1409 | AACACATGGCATAAATGAGTCC/TCTTCTTGTGAAGTCGCTGAA | This study |
| DNase I footprinting | ||
| VP1388 | TTGAATTTGTTAACTTCTGCC/ATTCTCCACAACCAACATCG | This study |
| VP1400 | GCGTGCTAATCATTCTTTG/ACACTTTGCACTCTTTTATGG | This study |
| GCGTGCTAATCATTCTTTG/ACACTTTGCACTCTTTTATGG | This study | |
| VP1409 | ATTAGGAACAAAGATCACACA/CGCTTGGATTACTTTCATCG | This study |
| AAGTTTATTAAAATGTTC/GCCTGCTATGCCAAATGA | This study | |
| GAACAAAGATCACACAAAATGGA/CGCTTGGATTACTTTCATCG | This study | |