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. 2017 Oct 16;8:1334. doi: 10.3389/fimmu.2017.01334

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Copyright © 2017 Liao, Xue, Zhao, Li, Liu, Chen, Brand, Zheng, Zhang, Zheng and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Skin presensitization before kidney transplantation induces acute antibody-mediated rejection. Renal allografts were obtained at 5 days post operation. (A) Survival of allografts from donors; (B) levels of antidonor-specific antibodies (IgG and IgM) before operation and at 5 days post kidney transplantation (KT5) by immunofluorescence and flow cytometry. Date are expressed as mean fluorescence intensity; (C) histological changes evaluated by the semiquantitative Banff scoring criteria: 0, absent; 1, mild; 2, moderate; and 3, prominent; (D) histologic evaluation of renal allografts posttransplant. Hematoxylin and eosin staining showing interstitial vasculitis, hemorrhage, and edema. Periodic acid-Schiff (PAS) staining showing tubular necrosis. Anti-C3d antibody and anti-IgG antibody staining showing the deposition of C3d and IgG (*p < 0.05, **p < 0.01).