Figure 3.

iTreg treatment reduces CD4⁺ cells including Th1, Th17, and Tfh infiltration in renal grafts. Renal allografts were obtained at 5 days post operation. (A) CD4⁺ cells among CD45⁺ cells; (B) relative percentages of CD4⁺ cells among the CD45⁺ cells; (C) absolute number of CD4⁺ cells; (D,E) IFN-γ⁺ cells among the CD4⁺ cells; (F) CD4⁺IFN-γ⁺ cells; (G,H) IL-17A⁺ cells among the CD4⁺ cells; (I) CD4⁺IL-17A⁺ cells; (J,K) CXCR5⁺ cells among the CD4⁺CD278⁺PD-1⁺ cells; (L) CD4⁺CD278⁺PD-1⁺CXCR5⁺ cells. Data are shown as mean ± SD of four transplants (*p < 0.05, ***p < 0.001).