Figure 6.

CD11c^high dendritic cell depletion at later time points has no impact on T cell activation and parasite burden. RosaiDTR/CD11c-cre (TG) and RosaiDTR wild-type (WT) mice were infected with Plasmodium yoelii and treated daily with DT (+DT) starting at day 4 post infection. At day 7 and day 14 post infection splenocytes were isolated. The percentage of (A) IFN-γ producing CD8⁺ and CD4⁺ T cells, (B) GzmB-expressing CD8⁺ T cells and CD69-expressing CD4⁺Foxp3⁻ T cells as well as (C) the percentage of Foxp3⁺ CD4⁺ regulatory T cells (Tregs) were determined by flow cytometry. Representative dot plots are shown in the upper panels. Results from two to four independent experiments with n = 7–14 mice per time point were summarized as mean ± SEM. (D) Parasitemia was determined at days 3, 5, 7, 10, 12, and 14 post infection by Giemsa-staining and results from two to four independent experiments with n = 23–24 mice in total are depicted as mean ± SEM. Statistical analysis was performed using the Student’s t-test and One-Way ANOVA (D) with *p < 0.05; ***p < 0.001.