Figure 7.

A single diphtheria toxin (DT)-injection 1 day before Plasmodium yoelii infection is sufficient to restrict dendritic cells (DC)-mediated T cell activation. RosaiDTR/CD11c-cre (TG) and RosaiDTR wild-type (WT) mice were treated once with DT (+DT) 1 day before infection with P. yoelii. (A) The percentage of CD11c^high DCs in uninfected DT-treated RosaiDTR/CD11c-cre mice was analyzed from days 1 to 4 after DT treatment in the spleen by flow cytometry. Representative dot plots are shown. At day 3 post infection (B), the frequencies of CD69-expressing CD8⁺, CD4⁺Foxp3⁻ T cells, and CD4⁺Foxp3⁺ Tregs were determined by flow cytometry, (C) the amount of IFN-γ and TNF-α was measured in the serum by Luminex technology, and (D) parasitemia was determined by Giemsa-staining. Results from two independent experiments with n = 8 mice were summarized as mean ± SEM. Student’s t-test test was used for statistical analysis. *p < 0.05; **p < 0.001.