Figure 4.

1,25-Dihydroxyvitamin D [1,25(OH)₂D₃] increases LPS-induced hemeoxygenase-1 (HO-1) expression in macrophages. (A,B) LPS-induced HO-1 mRNA expressions in bone marrow-derived macrophages (BMDMs) with 1,25(OH)₂D₃ (A) various dose treatment (2, 20, and 200 nM) or (B) time course (0, 2, 4, 6, 8, and 10 h) were detected by QPCR normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01 versus control, p < 0.0001 versus LPS alone. (C,D) The LPS-induced HO-1 protein expression in BMDMs in absent or present of 1,25(OH)₂D₃ (C) various dose (0.2, 2, 20, and 200 nM) or (D) time course (0, 4, 8, and 16 h) treatment. (E) The protein expression of HO-1 with 1,25(OH)₂D₃ time course (0, 4, 8, 16, and 32 h) treatment in BMDMs. Band intensities were quantified from three independent experiments (C–E). Data are represented as mean ± SD. **p < 0.01 versus control, p < 0.0001 versus LPS alone.